c-Jun Monoclonal Antibody

Target Information

Target Name	c-Jun
Uniprot ID	P05412
Cellular Localization	Nucleus.
Immunogen	Synthetic peptide.
Function	Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Product Details

Description	Rabbit monoclonal antibody to c-Jun.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Rat, Human
Immunogen	Synthetic peptide.
Positive Control	WB: HEK-293, MOJ/3T3 and PC-12 cell lysates. IHC-P: Rat liver, mouse cerebrum and human cervix carcinoma tissues. ICC/IF: NIH/3T3 and HeLa cells. ICC/IF KO: HEK293 cells (HEK293-JUN KO cells used as a negative cell line). Flow Cyt (Intra): HEK-293 cells IP: NIH/3T3 cell lysate
Application	Flo, ICC/IF, IHC-P, IP, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.20 Preservative: 0.01% Sodium Azide Constituent: 40% Glycerol, 59% PBS, 0.05% BSA
Handling	Avoid freeze-thaw cycles.