c-Kit (phospho Y703) Polyclonal Antibody

Target Information

Target Name	c-Kit (phospho Y703)
Uniprot ID	P10721
Cellular Localization	Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
Immunogen	Synthetic peptide corresponding to Human c-Kit (phospho Y703).
Function	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Product Details

Description	Rabbit polyclonal antibody to c-Kit (phospho Y703).
Host Species	Rabbit
Clonality	Polyclonal
Purity	Immunogen affinity purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Synthetic peptide corresponding to Human c-Kit (phospho Y703).
Positive Control	WB: HepG2 treated with EGF cell lysate. Colon cancer lysate. U2OS lysate. Renal cancer cell line lysate.
Application	WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Store at -20 °C
Storage Buffer	pH: 7 Preservative: 0.02% Sodium Azide Constituent: PBS, 50% Glycerol, 0.87% Sodium Chloride
Handling	Avoid freeze-thaw cycles.