CCL4/MIP-1 Beta Monoclonal Antibody

Target Information

Target Name	CCL4/MIP-1 Beta
Uniprot ID	P13236
Cellular Localization	Secreted.
Immunogen	Recombinant fragment.
Function	Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Product Details

Description	Rabbit monoclonal antibody to CCL4/MIP-1 beta.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Human
Immunogen	Recombinant fragment.
Positive Control	ICC/IF: THP-1 cells (treated with PMA, 100nM for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 µg/ml) for another 3 hours.). Flow Cyt (Intra): THP-1 (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) and RAW 264.7 cells (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours).
Application	Dot, Flo, ICC/IF

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	Preservative: 0.01% Sodium Azide Constituent: 59.94% PBS, 40% Glycerol, 0.05% BSA
Handling	Avoid freeze-thaw cycles.