Cat. No.: IBDA-431730
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Online InquiryTarget Information
| Target Name | CD163 |
| Uniprot ID | Q86VB7 |
| Cellular Localization | Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. |
| Immunogen | Recombinant fragment. |
| Function | Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. |
Product Details
| Description | Rat monoclonal antibody to CD163 - Chimeric – BSA and Azide Free. |
| Host Species | Rat |
| Clonality | Monoclonal |
| Purity | Ion Exchange Chromatography |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Rat, Human |
| Immunogen | Recombinant fragment. |
| Positive Control | WB: Whole cell lysates: SU-DHL-1 (human large cell lymphoma histiocytic), THP-1 (human monocytic leukemia monocyte), U937 (human histiocytic lymphoma monocyte). Mouse and rat spleen tissue lysates. IHC-P: Human tonsil, liver, and placenta tissues. Mouse and rat spleen tissues. |
| Application | IHC-P, WB |
Storage & Handling
| Shipping | Shipped at 4 °C. |
| Storage | Store at 4 °C |
| Storage Buffer | pH: 7.2 Constituent: 100% PBS |
| Handling | Do not freeze. |