CD163 Monoclonal Antibody

Target Information

Target Name	CD163
Uniprot ID	Q86VB7
Cellular Localization	Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Immunogen	Recombinant full length protein corresponding to Human CD163.
Function	Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Product Details

Description	Mouse monoclonal antibody to CD163.
Host Species	Mouse
Clonality	Monoclonal
Purity	Protein G purified
Isotype	IgG1
Conjugation	Unconjugated
Reactivity	Human, African green monkey
Immunogen	Recombinant full length protein corresponding to Human CD163.
Positive Control	WB: HEK-293T cell lysate transfected with pCMV6-ENTRY CD163 cDNA. IHC-P: Human liver tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY CD163.
Application	ICC/IF, IHC-P, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Upon delivery aliquot. Store at -20 °C
Storage Buffer	pH: 7.30 Preservative: 0.02% Sodium Azide Constituent: 50% Glycerol, PBS, 1% BSA
Handling	Avoid freeze-thaw cycles.