CD163 Monoclonal Antibody

Target Information

Target Name	CD163
Uniprot ID	Q86VB7
Cellular Localization	Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Immunogen	Human monocytes (epitope recognized SRCR domain 9).
Function	Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Product Details

Description	Mouse monoclonal antibody to CD163.
Host Species	Mouse
Clonality	Monoclonal
Isotype	IgG1
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Human monocytes (epitope recognized SRCR domain 9).
Positive Control	Flow Cyt (Intra): THP-1 cells. IHC-Fr: Human tonsil tissue.
Application	Flo, IHC-Fr

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Upon delivery aliquot. Store at -20 °C
Storage Buffer	Preservative: 0.02% Sodium Azide Constituent: PBS, 0.1% BSA
Handling	Avoid freeze-thaw cycles.