Complement C3/C3b Monoclonal Antibody

Target Information

Target Name	Complement C3/C3b
Synonyms	Breast Epithelial Mucin | BEM
Immunogen	Purified human iC3b.

Product Details

Description	C3b antibody is an unconjugated mouse monoclonal antibody to human C3b (Complement C3).
Host Species	Mouse
Clonality	Monoclonal
Purity	Protein A purified
Conjugation	Unconjugated
Modifications	Unmodified
Reactivity	Human
Specificity	Recognizes 42kD human inactive complement component 3b (iC3b) neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognized by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosus and Rheumatoid arthritis.
Immunogen	Purified human iC3b.
Application	ELISA, Flo, IHC, IHC-Fr, IHC-P, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	BBS, 0.09% Sodium Azide
Handling	Avoid freeze-thaw cycles.