Cytosolic Phospholipase A2 Assay Kit

Overview

Sample Type	Cell culture extracts, Tissue Extracts
Detection Method	Colorimetric
Assay Type	Enzyme activity (quantitative)
Product Type	Macrophage / Inflamm.
Species Reactivity	Reacts with: Mammals, Other species
Platform	Microplate reader

Target

Function	Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Tissue Specificity	Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
Sequence Similarities	Contains 1 C2 domain. Contains 1 PLA2c domain.
Cellular Localization	Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
Domain	The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	pH: 7.2 Constituent: 100% PBS