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ECT2 Polyclonal Antibody

Cat. No.: IBDA-430874

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Target Information

Target Name ECT2
Synonyms ECT2 | ARHGEF31 | Protein ECT2
Uniprot ID Q9H8V3
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.

Product Details

Description ECT2 antibody is an unconjugated rabbit polyclonal antibody to ECT2 (pThr790) from human.
Host Species Rabbit
Clonality Polyclonal
Purity Affinity purified
Conjugation Unconjugated
Modifications Unmodified
Reactivity Chicken, Zebrafish, Rat, Mouse, Dog, Human, Chimpanzee
Specificity Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity. A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.
Application ELISA, IP, WB

Storage & Handling

Shipping Shipped at 4 °C.
Storage Short term: 4 °C. Long term: -20 °C
Storage Buffer 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Handling Avoid freeze-thaw cycles.
! For research use only, not intended for any clinical use.