FAT10 Polyclonal Antibody

Target Information

Target Name	FAT10
Uniprot ID	O15205
Cellular Localization	Nucleus. Cytoplasm. Accumulates in aggresomes under proteasome inhibition conditions.
Immunogen	Recombinant full length protein within Human FAT10 aa 1 to the C-terminus.
Function	Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).

Product Details

Description	Mouse polyclonal antibody to FAT10.
Host Species	Mouse
Clonality	Polyclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Recombinant full length protein within Human FAT10 aa 1 to the C-terminus.
Positive Control	FAT10 transfected 293T cell lysate. HeLa cells.
Application	ICC/IF, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.4 Constituent: 100% PBS
Handling	Avoid freeze-thaw cycles.