Human Azurocidin ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	3 h 30 min
Assay Duration	Multiple steps standard assay
Product Type	Macrophage / Inflamm.
Sensitivity	< 10 pg/ml
Range	62.5 pg/ml - 4000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
Sequence Similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.
Cellular Localization	Cytoplasmic granule. Cytoplasmic granules of neutrophils.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.