Human FAS ELISA Kit (CD95)

Overview

Sample Type	Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard assay
Product Type	Cell Type Markers
Sensitivity	< 3 pg/ml
Range	31.2 pg/ml - 2000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Microplate

Target

Function	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue Specificity	Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Sequence Similarities	Contains 1 death domain. Contains 3 TNFR-Cys repeats.
Cellular Localization	Secreted and Cell membrane.
Domain	Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	pH: 7.2 Constituent: 100% PBS