Human GRX2 ELISA Kit

Overview

Sample Type	Cell Lysate
Detection Method	Colorimetric
Assay Type	Sandwich (qualitative)
Assay Time	4 h
Assay Duration	Multiple steps standard assay
Product Type	Redox Metabolism
Sensitivity	0.3 ng/ml
Range	0.038 ng/ml - 2.4 ng/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
Tissue Specificity	Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
Sequence Similarities	Belongs to the glutaredoxin family. Contains 1 glutaredoxin domain.
Cellular Localization	Mitochondrion and Nucleus.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.