Human IRAK-1 Matched Antibody Pair Kit

Overview

Detection Method	Colorimetric
Assay Type	ELISA set
Product Type	TLR Signaling
Sensitivity	2.5 pg/ml
Range	62.5 pg/ml - 4000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Reagents
Application	ELISA

Target

Function	Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Tissue Specificity	Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Sequence Similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. Contains 1 protein kinase domain.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS