Human Neprilysin ELISA Kit

Overview

Sample Type	Cell culture supernatant, Urine
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Cell Type Markers
Sensitivity	97.2 pg/ml
Range	156 pg/ml - 10000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence Similarities	Belongs to the peptidase M13 family.
Cellular Localization	Cell membrane.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.