Human Nrf2 ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard assay
Product Type	Redox Metabolism
Sensitivity	14 pg/ml
Range	13.72 pg/ml - 10000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Tissue Specificity	Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence Similarities	Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain.
Cellular Localization	Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Domain	Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS