IKB Alpha Monoclonal Antibody

Target Information

Target Name	IKB Alpha
Uniprot ID	P25963
Cellular Localization	Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Immunogen	Synthetic peptide.
Function	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Product Details

Description	Rabbit monoclonal antibody to IKB alpha.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Rat, Human
Immunogen	Synthetic peptide.
Positive Control	WB: Wild type HAP1 whole cell lysate. HeLa whole cell lysate. PC-12, SH-SY5Y, HepG2, K562, NIH/3T3 and RAW 264.7 cell lysate. Human fetal liver lysate. IHC-P: Human prostate carcinoma and stomach tissue. Rat and mouse kidney tissue. ICC/IF: HeLa and RAW 264.7 cells. IP: IKB alpha IP in HeLa cell lysate. PC-12 whole cell lysate. Flow Cyt (Intra): HeLa cells.
Application	Flo, ICC/IF, IHC-P, IP, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.20 Preservative: 0.01% Sodium Azide Constituent: PBS, 40% Glycerol, 0.05% BSA
Handling	Avoid freeze-thaw cycles.