IRF3 Monoclonal Antibody

Target Information

Target Name	IRF3
Uniprot ID	Q14653
Cellular Localization	Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Immunogen	Synthetic peptide within Human IRF3 aa 50-150.
Function	Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Product Details

Description	HRP Rabbit monoclonal antibody to IRF3.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	HRP
Reactivity	Human
Immunogen	Synthetic peptide within Human IRF3 aa 50-150.
Positive Control	WB: HeLa, Jurkat and wild-type HAP1 whole cell lysates. IHC-P: Normal human tonsil tissue.
Application	IHC-P, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C. Store in the dark
Storage Buffer	pH: 7.40 Preservative: 0.1% Proclin 300 Solution Constituent: PBS, 30% Glycerol, 1% BSA
Handling	Avoid freeze-thaw cycles.