IRF3 Monoclonal Antibody

Target Information

Target Name	IRF3
Uniprot ID	Q14653
Cellular Localization	Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Immunogen	Synthetic peptide within Human IRF3 aa 50-150.
Function	Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Product Details

Description	Rabbit monoclonal antibody to IRF3.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Human
Immunogen	Synthetic peptide within Human IRF3 aa 50-150.
Positive Control	WB: A549, HeLa, Jurkat, THP-1, Daudi, HepG2 whole cell lysates. Human fetal heart and kidney lysates. Mouse heart and spleen lysates, NIH/3T3 whole cell lysates. IHC-P: Human tonsil, Human squamous cell carcinoma of cervix, Mouse spleen. ICC/IF: HeLa cells Flow: U937 cells, HAP1-wt cells.
Application	Flo, ICC/IF, IHC-P, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.2 Preservative: 0.01% Sodium Azide Constituent: 40% Glycerol, 0.05% BSA, 59% PBS
Handling	Avoid freeze-thaw cycles.