IRF3 Monoclonal Antibody

Target Information

Target Name	IRF3
Uniprot ID	Q14653
Cellular Localization	Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Immunogen	Recombinant fragment: VAWVNKSRTR FRIPWKHGLR QDAQQEDFGI FQAWAEATGA YVPGRDKPDL PTWKRNFRSA LNRKEGLRLA EDRSKD, corresponding to amino acids 24-150 of Human IRF3.
Function	Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Product Details

Description	Mouse monoclonal antibody to IRF3.
Host Species	Mouse
Clonality	Monoclonal
Purity	Tissue culture supernatant
Isotype	IgG1
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Recombinant fragment: VAWVNKSRTR FRIPWKHGLR QDAQQEDFGI FQAWAEATGA YVPGRDKPDL PTWKRNFRSA LNRKEGLRLA EDRSKD, corresponding to amino acids 24-150 of Human IRF3.
Positive Control	HEK293 whole cell lysate RIPA lysate of HeLa cells Hela cells
Application	Flo, ICC/IF, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.40 Preservative: 0.05% Sodium Azide Constituent: 0.316% Tris HCl, 1% BSA, 1.16% Sodium Chloride
Handling	Avoid freeze-thaw cycles.