Cat. No.: IBDA-431892
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Online InquiryTarget Information
| Target Name | IRF3 (phospho S386) |
| Uniprot ID | Q14653 |
| Cellular Localization | Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. |
| Immunogen | Synthetic peptide corresponding to Human IRF3 (phospho S386). |
| Function | Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. |
Product Details
| Description | Rabbit monoclonal antibody to IRF3 (phospho S386). |
| Host Species | Rabbit |
| Clonality | Monoclonal |
| Purity | Protein A purified |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to Human IRF3 (phospho S386). |
| Positive Control | WB: MCF7 cells treated with Calyculin A. |
| Application | Dot, WB |
Storage & Handling
| Shipping | Shipped at 4 °C. |
| Storage | Short term: 4 °C. Long term: -20 °C |
| Storage Buffer | pH: 7.20 Preservative: 0.01% Sodium Azide Constituent: PBS, 40% Glycerol, 0.5% BSA |
| Handling | Avoid freeze-thaw cycles. |