IRF3 Polyclonal Antibody

Target Information

Target Name	IRF3
Uniprot ID	Q14653
Cellular Localization	Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Immunogen	Synthetic peptide within Human IRF3 aa 50-150 conjugated to keyhole limpet haemocyanin.
Function	Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Product Details

Description	Rabbit polyclonal antibody to IRF3.
Host Species	Rabbit
Clonality	Polyclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Human
Immunogen	Synthetic peptide within Human IRF3 aa 50-150 conjugated to keyhole limpet haemocyanin.
Positive Control	Mouse stomach tissue. Human kidney and colon carcinoma tissues.
Application	IHC-P

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.2 Preservative: 0.09% Sodium Azide Constituent: 50% Glycerol, 1% BSA
Handling	Avoid freeze-thaw cycles.