JNK1 + JNK2 + JNK3 (phospho T183 + Y185) Polyclonal Antibody

Target Information

Target Name	JNK1 + JNK2 + JNK3 (phospho T183 + Y185)
Uniprot ID	P45983
Cellular Localization	Cytoplasm. Nucleus.
Immunogen	Synthetic peptide corresponding to Human JNK1 + JNK2 + JNK3 aa 150-250 (phospho T183 + Y185).
Function	Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Product Details

Description	Rabbit polyclonal antibody to JNK1 + JNK2 + JNK3 (phospho T183 + Y185).
Host Species	Rabbit
Clonality	Polyclonal
Purity	Immunogen affinity purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Synthetic peptide corresponding to Human JNK1 + JNK2 + JNK3 aa 150-250 (phospho T183 + Y185).
Application	WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Store at -20 °C
Storage Buffer	pH: 7.40 Preservative: 0.02% Sodium Azide Constituent: PBS, 50% Glycerol, 0.87% Sodium Chloride Without Mg2+ and Ca2+
Handling	Avoid freeze-thaw cycles.