JNK1 (phospho T183 + Y185) Monoclonal Antibody

Target Information

Target Name	JNK1 (phospho T183 + Y185)
Immunogen	Synthetic peptide.
Function	Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Product Details

Description	Rabbit monoclonal antibody to JNK1 (phospho T183 + Y185).
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Synthetic peptide.
Positive Control	WB: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes and U-2 OS treated with 50 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours cell lysate. Dot Blot: JNK1 (phospho T183/Y185) peptide. IP: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes cell lysate.
Application	Dot, IP, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.2 Preservative: 0.01% Sodium Azide Constituent: PBS, 40% Glycerol, 0.05% BSA
Handling	Avoid freeze-thaw cycles.