Lck Monoclonal Antibody

Target Information

Target Name	Lck
Uniprot ID	P06239
Cellular Localization	Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
Immunogen	Recombinant fragment.
Function	Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.

Product Details

Description	Rabbit monoclonal antibody to Lck.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Rat, Human
Immunogen	Recombinant fragment.
Positive Control	WB: Raji, Ramos, Jurkat and WEHI-231 whole cell lysates. human thymus and tonsil tissue lysates. mouse thymus and lymph node tissue lysates. rat thymus tissue lysate. IHC-P: Human colon tissue. mouse and rat spleen tissues. ICC/IF: Ramos and WEHI-231 cells. Flow Cyt (Intra): Jurkat and WEHI-231 cells.
Application	Flo, ICC/IF, IHC-P, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.2 Preservative: 0.01% Sodium Azide Constituent: 0.05% BSA, PBS, 40% Glycerol
Handling	Avoid freeze-thaw cycles.