Cat. No.: IBDA-434714
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Online InquiryTarget Information
| Target Name | Lck (phospho Y505) |
| Uniprot ID | P06239 |
| Cellular Localization | Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form. |
| Immunogen | Synthetic peptide within Human Lck (phospho Y505). |
| Function | Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3. |
Product Details
| Description | PE Rabbit monoclonal antibody to Lck (phospho Y505). |
| Host Species | Rabbit |
| Clonality | Monoclonal |
| Purity | Protein A/G purified |
| Isotype | IgG |
| Conjugation | PE |
| Reactivity | Human |
| Immunogen | Synthetic peptide within Human Lck (phospho Y505). |
| Positive Control | Flow Cyt: Daudi cells treated with IFNa plus IL4 |
| Application | Flo |
Storage & Handling
| Shipping | Shipped at 4 °C. |
| Storage | Store at 4 °C. Store in the dark |
| Storage Buffer | Preservative: 0.09% Sodium Azide Constituent: 99.71% PBS, 0.2% BSA |