Macrophage Inflammatory Protein 1 Alpha/CCL3 Monoclonal Antibody

Target Information

Target Name	Macrophage Inflammatory Protein 1 Alpha/CCL3
Uniprot ID	P10147
Cellular Localization	Secreted.
Immunogen	Recombinant full length protein.
Function	Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Product Details

Description	Rabbit monoclonal antibody to MIP-1 alpha/CCL3.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse
Immunogen	Recombinant full length protein.
Positive Control	WB: RAW 264.7 treated with 100 ng/ml LPS for 3 hours and then 300 ng/ml Brefeldin A was added for the last 3 hours whole cell lysate. ICC/IF: RAW 264.7 cells treated with 100 ng/ml Lipopolysaccharide for 3h, followed by 300 ng/ml Brefeldin A for 3h. Flow Cyt (Intra):RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3h, then add 300 ng/ml Brefeldin A for the last 3h. IP: RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3h, then add 300 ng/ml Brefeldin A for the last 3h whole cell lysate.
Application	EIA, Flo, ICC/IF, IP, sELISA, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	Preservative: 0.01% Sodium Azide Constituent: PBS, 40% Glycerol, 0.05% BSA
Handling	Avoid freeze-thaw cycles.