MIF Monoclonal Antibody

Target Information

Target Name	MIF
Uniprot ID	P14174
Cellular Localization	Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
Immunogen	Synthetic peptide within Human MIF aa 50 to the C-terminus (Cysteine residue).
Function	Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Product Details

Description	Rabbit monoclonal antibody to MIF.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Rat, Human
Immunogen	Synthetic peptide within Human MIF aa 50 to the C-terminus (Cysteine residue).
Positive Control	WB: Y79, THP-1, and Molt-4 cell lysates. Mouse and rat brain tissue lysates. Flow Cyt (Intra): THP-1, and Molt-4 cells. IP: Human fetal brain tissue lysate.
Application	Flo, IP, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	Preservative: 0.01% Sodium Azide Constituent: 40% Glycerol, 59% PBS, 0.05% BSA
Handling	Avoid freeze-thaw cycles.