Cat. No.: IBDA-433973
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Online InquiryTarget Information
| Target Name | MIF |
| Uniprot ID | P14174 |
| Cellular Localization | Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. |
| Immunogen | Recombinant full length protein. |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
Product Details
| Description | Rabbit monoclonal antibody to MIF. |
| Host Species | Rabbit |
| Clonality | Monoclonal |
| Purity | Protein A purified |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Rat, Human |
| Immunogen | Recombinant full length protein. |
| Positive Control | WB: Human fetal brain and fetal spleen lysates. Mouse brain and kidney lysate. Rat brain lysate. J774A.1, Y79, Jurkat, NIH/3T3, RAW 264.7, C6 and Neuro-2a whole cell lysates. ICC/IF: Neuro-2a and RAW 264.7 cells. Flow Cyt (Intra): Neuro-2a and RAW 264.7 cells. |
| Application | Flo, ICC/IF, WB |
Storage & Handling
| Shipping | Shipped at 4 °C. |
| Storage | Short term: 4 °C. Long term: -20 °C |
| Storage Buffer | pH: 7.2 Preservative: 0.01% Sodium Azide Constituent: 40% Glycerol, 0.05% BSA, PBS |
| Handling | Avoid freeze-thaw cycles. |