MIF Polyclonal Antibody

Target Information

Target Name	MIF
Uniprot ID	P14174
Cellular Localization	Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
Immunogen	Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF.
Function	Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Product Details

Description	Chicken polyclonal antibody to MIF.
Host Species	Chicken
Clonality	Polyclonal
Purity	IgY fraction
Isotype	IgY
Conjugation	Unconjugated
Reactivity	Recombinant fragment
Immunogen	Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF.
Positive Control	Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).
Application	WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	Preservative: 0.01% Sodium Azide Constituent: 0.42% Potassium Phosphate, 0.87% Sodium Chloride
Handling	Avoid freeze-thaw cycles.