MIF Polyclonal Antibody

Target Information

Target Name	MIF
Synonyms	MIF | GLIF | L-dopachrome tautomerase | L-dopachrome isomerase | MMIF | Phenylpyruvate tautomerase
Uniprot ID	P14174
Immunogen	This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.

Product Details

Description	MIF antibody is an unconjugated chicken polyclonal antibody to human MIF.
Host Species	Chicken
Clonality	Polyclonal
Purity	Selective precipitation and salt fractionation followed by daialysis.
Conjugation	Unconjugated
Modifications	Unmodified
Reactivity	Human
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F(c) domain of the chicken IgG molecule may interact with cells non-specifically.
Immunogen	This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
Application	ELISA, WB

Storage & Handling

Shipping	Shipped on dry ice.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Handling	Avoid freeze-thaw cycles.