NF-κB Luciferase Reporter Raw 264.7 Cell Line

Product Details

Synonyms	NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb 264.7, 264.7 raw, NF-kB, nfkb, nf-kb
Description	NF-κB Luciferase Reporter Raw 264.7 Cell Line is a Raw 264.7 cell line expressing firefly luciferase driven by four copies of the NF-kB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line has been functionally validated and responds to TNFα and RANKL.
Host Cell Line	Raw 264.7, murine macrophage cell line, adherent.
Applications	• Screen for activators or inhibitors of the NF-κB signaling pathway. • Screen for activators or inhibitors of the RANKL/RANK pathway.
Supplied As	Each vial contains >1x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 11 • Growth Medium 11A Materials Used in the Cellular Assay • Mouse RANKL • Mouse TNFα • White clear-bottom 96-well cell culture plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only