NF-κB Reporter Cellular Assay Pack (HCT116)

Product Details

Synonyms	NF-κB Cell Line, NFkappaB Cell Line, Luciferase Reporter, NF-kB Cell Line, NFkB Cell Line, nfkb, nf-kb
Description	The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc) - HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine.
Materials Required But Not Supplied	• IL-1β • TNFα, human • IFN-gamma • IFN-alpha • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Stable at least 12 months from date of receipt, when stored as directed (-20°C).
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only