NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line

Product Details

Synonyms	NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb
Description	An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κBtranscription factor through luminescence readout.). This cell line responds to human cytokineIL-1β, responds moderately to human TNF, and does not respond to human IFN-λ (2 μg/ml). Reducing the amount of serum during incubation period may increase the sensitivity tocytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell lineprovides an excellent platform to enable exogenous expression of a protein of interest to studyits downstream effect on NF-κB signaling.
Host Cell Line	CHO-K1
Mycoplasma Testing	This cell line has been screened using the Mycoplasma Detection Kit to confirm the absence of Mycoplasma contamination.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Store in liquid nitrogen immediately upon receipt.
Shipping Temperature	dry ice
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only