NFAT Luciferase Reporter CHO Cell Line (PKC/Ca2+ Signaling Pathway)

Product Details

Description	The NFAT Luciferase Reporter CHO cell line (PKC/Ca2+ Signaling Pathway) contains a firefly luciferase gene under the control of NFAT response element stably integrated into CHO K1 cells. This cell line is validated for its response to phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin.
Host Cell Line	CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Applications	• Monitor NFAT stimulation • Screen for activators or inhibitors of the PKC/ Ca2+
Supplied As	Each vial contains 2 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 3 • Growth Medium 3 Materials Required for Cellular Assay • Assay Medium: Thaw medium 3 • Growth Medium 3D • PMA  • Ionomycin • 96-well tissue culture treated white clear-bottom assay plate  • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only