Cat. No.: IBDRGCL60461
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| Synonyms | Nuclear factor of activated T cells; T cell, PKC Ca 2+, Calcium PKC Pathway |
| Description | The NFAT Reporter – HEK293 cell line contains a firefly luciferase gene under the control of NFAT response element stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of phorbol 12-myristate 13-acetate (PMA) with ionomycin. |
| Host Cell Line | HEK293 |
| Supplied As | Each vial contains ~2 X 106 cells in 1 mL of 10% DMSO |
| Materials Required But Not Supplied | • PMA Prepare stock solution in DMSO. • Ionomycin Prepare stock solution in DMSO. • Assay medium: Thaw Medium 1 • Growth Medium 1B • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate • RO-31-8220 or other PKC inhibitor • Luminometer |
| Mycoplasma Testing | The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species. |
| Scientific Category | Cell Signaling Pathway |
Storage & Handling
| Storage/Stability | Immediately upon receipt, store in liquid nitrogen. |
| Shipping Temperature | -80°C |
| Warnings | Avoid freeze/thaw cycles |
| Regulatory Status | Research Use Only |