NLRP3 Monoclonal Antibody

Target Information

Target Name	NLRP3
Uniprot ID	Q96P20
Cellular Localization	Cytoplasm.
Immunogen	Recombinant fragment.
Function	May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

Product Details

Description	Rabbit monoclonal antibody to NLRP3.
Host Species	Rabbit
Clonality	Monoclonal
Purity	Protein A purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Mouse, Rat, Human
Immunogen	Recombinant fragment.
Positive Control	WB: THP-1 and J774A.1 whole cell lysate. LPS treated RAW 264.7 whole cell lysate. Rat spleen lysate. Mouse Wt BMDCs treated with 50 ng/ml LPS, 3h. Human THP1 Wt cells treated with 200 ng/ml LPS, 3h. RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate. IP: RAW 264.7 and THP-1 whole cell lysate. Flow Cyt (Intra): THP-1 cells.
Application	Flo, I-ELISA, IP, WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Short term: 4 °C. Long term: -20 °C
Storage Buffer	pH: 7.2 Preservative: 0.01% Sodium Azide Constituent: PBS, 40% Glycerol, 0.05% BSA
Handling	Avoid freeze-thaw cycles.