Nrf2 Transcription Factor Assay Kit (Colorimetric)

Overview

Sample Type	Nuclear Extracts
Detection Method	Colorimetric
Assay Type	Semi-quantitative
Assay Time	3 h 30 min
Product Type	Redox Metabolism
Sensitivity	> 600 ng/well
Species Reactivity	Reacts with: Mouse, Rat, Human
Platform	Microplate reader

Target

Function	Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Tissue Specificity	Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence Similarities	Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain.
Cellular Localization	Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Domain	Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.