p38 MAPK alpha ELISA Kit

Overview

Sample Type	Cell Lysate, Tissue Homogenate
Detection Method	Colorimetric
Assay Type	Semi-quantitative
Assay Time	1 h
Assay Duration	One step assay
Product Type	TLR Signaling
Sensitivity	300 pg/ml
Range	0.781 ng/ml - 100 ng/ml
Species Reactivity	Reacts with: Mouse, Human Predicted to work with: Rat
Platform	Microplate

Target

Function	Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue Specificity	Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence Similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Cellular Localization	Cytoplasm. Nucleus.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.