PD-1/IL-2 Luciferase Reporter Jurkat Cell Line

Product Details

Synonyms	Programmed cell death protein 1, Protein PD-1, hPD-1, CD279, PDCD1, PD1, Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, IL2
Description	PD-1/IL-2 Luciferase Reporter Jurkat Cell Line is a Jurkat T cell line constitutively expressing human PD-1 (Programmed Cell Death 1, also known as PDCD1, SLEB2, CD279, GenBank Accession #NM_005018), and conditionally expressing firefly luciferase under the control of a human interleukin-2 (IL-2) promoter. This cell line has been validated by flow cytometry for expression of PD-1, and in co-culture assays that assess the PD-L1/PD-1 interaction on Jurkat T cell activation.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	• Screen for inhibitors of PD-1 or PD-L1 signaling in a cellular model. • Characterize the biological activity of PD-1 and interaction with its ligands.
Supplied As	Each vial contains ˃1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2 • Growth Medium 2A Materials Required for Cellular Assay • PD-L1/TCR Activator CHO Cell Line • IL-2 Luciferase Reporter Jurkat Cell Line • Thaw Medium 3 • Anti-PD-1 Neutralizing Antibody • Anti-PD-L1 (CD274) Neutralizing Antibody • Anti-CD28 Agonist Antibody (Humanized) • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
UniProt	PD-1: Q15116; IL-2: P60568
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy Immune Checkpoints

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use an -80°C freezer for long-term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only