Phospho-AMPK alpha 1 (S487) and Total AMPK alpha 1 ELISA Kit

Overview

Sample Type	Cell Lysate
Detection Method	Colorimetric
Assay Type	Semi-quantitative
Assay Duration	Multiple steps standard assay
Product Type	Redox Metabolism
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Sequence Similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS