Phospho-p38 MAPK (Thr180+Tyr182) Translocation Assay Kit (Cell-Based)

Overview

Sample Type	Adherent cells, Suspension cells
Detection Method	Flow cytometry-fluorescent
Assay Type	Cell-based
Assay Duration	Multiple steps standard assay
Product Type	Macrophage / Inflamm.
Species Reactivity	Reacts with: Mouse, Rat, Human
Platform	Microplate (12 x 8 well strips)

Target

Function	Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue Specificity	Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence Similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Cellular Localization	Cytoplasm. Nucleus.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS