PPAR alpha Transcription Factor Assay Kit

Overview

Sample Type	Adherent cells, Suspension cells, Nuclear Extracts
Detection Method	Colorimetric
Assay Type	Semi-quantitative
Product Type	Redox Metabolism
Species Reactivity	Reacts with: Mouse, Rat, Human
Platform	Microplate reader

Target

Function	Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
Tissue Specificity	Skeletal muscle, liver, heart and kidney.
Sequence Similarities	Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Cellular Localization	Nucleus.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS