PPAR-gamma Ligand Screening/Characterization Assay Kit (Fluorometric)

Overview

Detection Method	Colorimetric/Fluorometric
Assay Type	Qualitative
Assay Duration	Multiple steps standard assay
Product Type	Redox Metabolism
Platform	Microplate
Application	Functional Studies

Target

Function	Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
Tissue Specificity	Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Sequence Similarities	Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Cellular Localization	Nucleus.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS