Cat. No.: IBDA-432344
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Online InquiryTarget Information
| Target Name | Rac1 |
| Uniprot ID | P63000 |
| Cellular Localization | Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Immunogen | Recombinant full length protein corresponding to Human Rac1. |
| Function | Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. |
Product Details
| Description | Rabbit polyclonal antibody to Rac1. |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Purity | Protein A purified |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Human |
| Immunogen | Recombinant full length protein corresponding to Human Rac1. |
| Positive Control | WB: HT-29, SW480, PC-3, LNCaP, HeLa, A431, Jurkat, 3T3L1 and 293T cell lysate. ICC: HeLa cells. IHC: Human breast, prostate and tonsil tissues. |
| Application | ICC, IHC-P, WB |
Storage & Handling
| Shipping | Shipped at 4 °C. |
| Storage | Upon delivery aliquot. Store at -20 °C |
| Storage Buffer | Preservative: 0.05% Sodium Azide Constituent: 0.1% BSA, 30% Glycerol, 69% PBS |
| Handling | Avoid freeze-thaw cycles. |