Rac1 Polyclonal Antibody

Target Information

Target Name	Rac1
Uniprot ID	P63000
Cellular Localization	Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunogen	Synthetic non-phosphopeptide derived from Human Rac1 around the phosphorylation site of serine 71 (P-L-SP-Y-P).
Function	Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Product Details

Description	Rabbit polyclonal antibody to Rac1.
Host Species	Rabbit
Clonality	Polyclonal
Purity	Immunogen affinity purified
Isotype	IgG
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Synthetic non-phosphopeptide derived from Human Rac1 around the phosphorylation site of serine 71 (P-L-SP-Y-P).
Positive Control	Extract from A431 cells treated with EGF.
Application	WB

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Store at -20 °C
Storage Buffer	pH: 7 Preservative: 0.02% Sodium Azide Constituent: 49% PBS, 50% Glycerol, 0.88% Sodium Chloride
Handling	Avoid freeze-thaw cycles.