RARα Luciferase Reporter HEK293 Cell Line

Product Details

Synonyms	RAR alpha, NCOR2, SMRT, RARA, Retinoic acid receptor alpha
Description	RARα Luciferase Reporter HEK293 Cell Line are engineered HEK293 cells expressing a conditional firefly luciferase reporter under the control of retinoid acid response elements (RARE), and constitutively expressing full length human Retinoic Acid Receptor alpha (RARα, NM_0.00964). This cell line is functionally validated to respond to all-trans retinoic acid (ATRA) stimulation.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Applications	• Monitor RARα-regulated pathway activity. • Screen RARα agonists and antagonists.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 6 • Growth Medium 6A Materials Required for Cellular Assay  • ATRA • Assay Medium 6A • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
UniProt	P10276
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Nuclear Receptor

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only