Recombinant Human EGFR Protein

Target Information

Sequence	LEEKKVCQGT KVCQGTSNKL GTSNKLTQLG KLTQLGTFED LGTFEDHFLS EDHFLSLQRM LSLQRMFNNC RMFNNCEVVL NCEVVLGNLE VLGNLEITYV LEITYVQRNY YVQRNYDLSF NYDLSFLKTI SFLKTIQEVA TIQEVAGYVL VAGYVLIALN VLIALNTVER LNTVERIPLE ERIPLENLQI LENLQIIRGN QIIRGNMYYE GNMYYENSYA YENSYALAVL YALAVLSNYD VLSNYDANKT YDANKTGLKE KTGLKELPMR KELPMRNLQE MRNLQEILHG QEILHGAVRF HGAVRFSNNP RFSNNPALCN NPALCNVESI CNVESIQWRD SIQWRDIVSS RDIVSSDFLS SSDFLSNMSM LSNMSMDFQN SMDFQNHLGS QNHLGSCQKC GSCQKCDPSC KCDPSCPNGS SCPNGSCWGA GSCWGAGEEN GAGEENCQKL ENCQKLTKII KLTKIICAQQ IICAQQCSGR QQCSGRCRGK GRCRGKSPSD GKSPSDCCHN SDCCHNQCAA HNQCAAGCTG AAGCTGPRES TGPRESDCLV ESDCLVCRKF LVCRKFRDEA KFRDEATCKD EATCKDTCPP KDTCPPLMLY PPLMLYNPTT LYNPTTYQMD TTYQMDVNPE MDVNPEGKYS PEGKYSFGAT YSFGATCVKK ATCVKKCPRN KKCPRNYVVT RNYVVTDHGS VTDHGSCVRA GSCVRACGAD RACGADSYEM ADSYEMEEDG EMEEDGVRKC DGVRKCKKCE KCKKCEGPCR CEGPCRKVCN CRKVCNGIGI CNGIGIGEFK GIGEFKDSLS FKDSLSINAT LSINATNIKH ATNIKHFKNC KHFKNCTSIS NCTSISGDLH ISGDLHILPV LHILPVAFRG PVAFRGDSFT RGDSFTHTPP FTHTPPLDPQ PPLDPQELDI PQELDILKTV DILKTVKEIT TVKEITGFLL ITGFLLIQAW LLIQAWPENR AWPENRTDLH NRTDLHAFEN LHAFENLEII ENLEIIRGRT IIRGRTKQHG RTKQHGQFSL HGQFSLAVVS SLAVVSLNIT VSLNITSLGL ITSLGLRSLK GLRSLKEISD LKEISDGDVI SDGDVIISGN VIISGNKNLC GNKNLCYANT LCYANTINWK NTINWKKLFG WKKLFGTSGQ FGTSGQKTKI GQKTKIISNR KIISNRGENS NRGENSCKAT NSCKATGQVC ATGQVCHALC VCHALCSPEG LCSPEGCWGP EGCWGPEPRD GPEPRDCVSC RDCVSCRNVS SCRNVSRGRE VSRGRECVDK RECVDKCNLL DKCNLLEGEP LLEGEPREFV EPREFVENSE FVENSECIQC SECIQCHPEC QCHPECLPQA ECLPQAMNIT QAMNITCTGR ITCTGRGPDN GRGPDNCIQC DNCIQCAHYI QCAHYIDGPH YIDGPHCVKT PHCVKTCPAG KTCPAGVMGE AGVMGENNTL GENNTLVWKY TLVWKYADAG KYADAGHVCH AGHVCHLCHP CHLCHPNCTY HPNCTYGCTG TYGCTGPGLE TGPGLEGCPT LEGCPTNGPK PTNGPKIPS PKIPS S—Linker(gggsgggs)—10*His(hhhhhhhhhh)
Sequence Similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.
Amino Acids	25 to 645
Cellular Localization	Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
Tissue Specificity	Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Function	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.

Product Details

Product Type	Protein
Species	Human
Source	HEK 293 Cells
Tag	His
Endotoxin Level	<1.0 Eu/µg
Protein Length	Protein fragment
Molecular Weight	71 kDa
Purity	>95%
Active	Yes
Animal free	No
Nature	Recombinant
Application	ELISA, FuncS, SDS-PAGE

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Store at -20 °C or -80 °C.
Handling	Avoid freeze / thaw cycle.