Cat. No.: IBDRGCL60489
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| Synonyms | SBE cell line, TGFbeta reporter, SMAD reporter, luciferase reporter, TGFβ, TGF-β, activin |
| Description | The TGFβ-Responsive SBE Reporter HEK293 Cell Line is a HEK293 cell line expressing Firefly luciferase reporter under the control of SMAD-responsive elements (SMAD binding elements, SBE). This cell line monitors the activity of the TGFβ (transforming growth factor beta)/SMAD Signaling Pathway. This cell line has been validated by activation in response to human TGFβ1 and to other cytokines of the TGFβ1 superfamily such as Activin A, GDF-8/Myostatin, and GDF-11/BMP-11. Luciferase activity induced by human TGFβ1 was decreased by SB525334, an inhibitor of the TGFβ1-receptor. |
| Host Cell Line | HEK293, Human Embryonic Kidney, epithelial-like cells, adherent |
| Applications | • Monitor activation of the TGFβ signaling pathway. • Screen for compounds that regulate the TGFβ/SMAD signaling pathway. |
| Supplied As | Each vial contains >1 x 106 cells in 1 mL of cell freezing medium |
| Materials Required But Not Supplied | Media Required for Cell Culture • Thaw Medium 1 • Growth Medium 1B Materials Used in Cellular Assay • Assay Medium 1B • Human TGFβ1 • Recombinant Human/Murine/Rat Activin A • Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein • Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein • Inhibitor SB525334 • Clear-bottom, white 96-well tissue culture-treated plate • Luminometer |
| UniProt | P01137, P61812, P10600, Q15796, P84022, Q13485 |
| Mycoplasma Testing | The cell line has been screened to confirm the absence of Mycoplasma species. |
| Scientific Category | Cell Signaling Pathway |
Storage & Handling
| Storage/Stability | Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. |
| Shipping Temperature | -80°C |
| Warnings | Avoid freeze/thaw cycles |
| Regulatory Status | Research Use Only |