Exploration of the Role of Tau in Stroke

Tau is a protein expressed in the adult brain. The function of tau is regulated by complex post-translational modifications, such as phosphorylation, glycosylation, acetylation, isomerization, nitration, and other modalities, so it plays different roles under different conditions. In stroke, tau regulates the development of disease through multiple pathways, including oxidative stress, apoptosis, autophagy, excitotoxicity, neuroinflammation, mitochondrial dysfunction, and other pathways. However, the exact mechanism of action is unknown to us. Therefore, Ace Therapeutics offers targeted services for the different functions of tau to accelerate your research exploring the role of tau in stroke while advancing the understanding of stroke. We offer different in vitro and in vivo stroke models for exploring the role of tau proteins in different pathological situations. We provide different quantitative proteomics research services for different post-translational modifications of tau proteins. In addition, we offer services to detect the expression level and localization of downstream genes or proteins of tau to complete your study.

Exploration of the Role of Phosphorylated Tau in Stroke

Phosphorylation modifications are one of the most common types of modifications that are involved in the regulation of a variety of cellular physiological processes such as signal transduction, cell structure, proliferation, and other processes. Therefore, understanding the role of phosphorylated tau in stroke is an integral part of exploring the role of tau in stroke.

  • Effective enrichment and purification of phosphorylated proteins is key to the study. We enrich phosphorylated proteins by immobilized metal ion affinity chromatography and titanium dioxide affinity chromatography.
  • We use TMT quantification, LFQ, DIA, and other mass spectrometry methods for quantitative detection.

Exploration of the Role of Glycosylated Tau in Stroke

Glycosylation plays an important role in determining protein structure and stability, but the role of glycosylated tau in stroke is unclear. Therefore, Ace Therapeutics provides a comprehensive service to explore the role of glycosylated tau in stroke.

  • Isolation and purification of glycosylated proteins by chromatography, affinity chromatography, and electrophoresis
  • Characterization of glycosylated proteins by UV spectrophotometry and electrochemistry
  • Quantification of glycosylated proteins by high performance liquid chromatography and mass spectrometry

Exploration of the Role of Acetylated Tau in Stroke

Acetylation modifications mainly regulate cellular transcriptional and metabolic pathways. Therefore, the study of acetylated tau is important to reveal the role of tau in stroke. Ace Therapeutics provides a mature technology platform to explore the role of acetylated tau in stroke.

  • SILAC-based quantitative acetylation modification analysis
  • iTRAQ-based quantitative acetylation modification analysis
  • Sequence analysis of the enriched acetylated peptides using LC-MS/MS

Workflow diagram to explore the mechanism of action of acetylated tau in stroke.Fig. 1 Workflow diagram to explore the mechanism of action of acetylated tau in stroke.

Ace Therapeutics offers a one-stop scientific service from protein extraction to data analysis to explore the role of tau proteins in stroke research in a scientific and accurate manner. We offer different assays for other post-translational modification regulations, such as isomerization, nitration, sumoylation, O-GlcNAcylation, and truncation. If you would like to learn more about our services, please feel free to contact us.

References
  1. Pluta, R., et al., Tau protein dysfunction after brain ischemia. J Alzheimers Dis, 2018. 66(2): p. 429-437.
  2. Chen, X.Jiang, H., Tau as a potential therapeutic target for ischemic stroke. Aging (Albany NY), 2019. 11(24): p. 12827-12843.
All of our services are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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